Multidrug resistant strains of Acinetobacter baumannii (MDR-AB) have emerged as alarming nosocomial pathogens among patients admitted to Intensive Care Unit and burned patients. The aim of this study was to determine the susceptibility of A. baumannii isolates, the carbapenems resistance patterns bla ^OXA-23 and IS_Aba elements of A. baumannii isolates among burned patients in Sari, Iran. During the course of this investigation, our aim was to examine prevalence and Expression of ade A and ade R Efflux Pump genes in Acinetobacter baumannii Strains resistant to Aminoglycosides by PCR and RT-PCR methods. Multidrug resistant strains of Acinetobacter baumannii (MDR-AB) have emerged as an important cause of hospital acquired infection among patients admitted to Intensive Care Unit and burned patients.A total of 100 isolates were identified as Acinetobacter baumannii from patients that were admitted to Intensive Care Unit and burned patients in Sari ‚ (Iran). During 2013, the isolates were tested for the determination of resistance to Aminoglycosides by Disk Diffusion method according to the CLSI protocol. On the next step ‚ we detected ade A and ade R Efflux Pumps gene in Acinetobacter baumannii that were resistant to Aminoglycosides by PCR method, Finally we Investigated Expression of ade A and ade R Efflux Pump genes by RT-PCR method. Among the 62 (62%) strains that showed resistance to Aminoglycosides, in 52 (83%) strains, we detected Ade A and Ade R genes. After RT-PCR was found that only in 3 strains of Acinetobacter baumannii ade A gene, and in 6 strains ade R gene have not been expressed.