Hepatitis C virus (HCV) infection is a health problem all over the world. It is a major reason of cirrhosis and hepatocellular carcinoma. In this study, we aimed to examine the relationship between liver Hepatic Activity Index (HAI) and Tumor Necrosis Factor alpha (TNF alpha) levels in patients with chronic hepatitis C, and to determine whether there is a significant correlation. Thirty five chronic HCV patients, who were monitored by Infection Diseases and Clinic Microbiology Clinic, were taken into the study. Liver Biopsy samples were examined by the same pathologist and necroinflammatory activity was evaluated according to Knodell’s classification. As the control group, 35 healthy volunteers without HCV infections were selected. TNF alpha was measured by ELISA method, for the serums acquired from bloods of patients and controls. TNF alpha and HAI levels was found to correlate in a positive, medium level, (r=0.379, p=0.02). In our study, we found a statistically significant relationship between HAI and TNF alpha levels in patients with chronic hepatitis C. We presume that serum TNF alpha level can be used to estimate the inflammation in the liver during HCV infection.