ISSN: 0973-7510

E-ISSN: 2581-690X

Open Access
Hafsan Hafsan1 , Nurhikmah Nurhikmah1, Yuniar Harviyanti1, Eka Sukmawati1, Isna Rasdianah1, Cut Muthiadin1, Laily Agustina2, Asmuddin Natsir2 and Ahyar Ahmad2
1Department of Biology, Faculty of Science and Technology, Universitas Islam Negeri Alauddin Makassar, Gowa, 92113, South Sulawesi, Indonesia.
2Department of Nutrition and Fodder of Faculty of Animal Husbandry, Universitas Hasanuddin, Makassar 90245, Indonesia.
J Pure Appl Microbiol. 2018;12(3):1277-1280
https://doi.org/10.22207/JPAM.12.3.29 | © The Author(s). 2018
Received: 09/04/2018 | Accepted: 21/06/2018 | Published: 30/09/2018
Abstract

This study was aimed at isolating endophytic bacteria from Zea mays L. that have potential as phytase enzyme producers. Isolation and screening were performed using the Phytase Selective Media (PSM) medium. Bacteria that grew and formed a clear zone around the colony on the media were indicated as phytase-producer bacteria. The isolates with the highest phytanic index were further characterized based on morphology and physiological properties, then molecularly identified based on a 16S-rRNA analysis.  Based on the results of Zea mays L. endophytic bacterial screening, bacterial isolate HF.7, was shown to be qualitatively able to produce phytase and can be developed as a phytase producer. Based on morphological and biochemical characterization, HF.7 belonged to the Burkholderia genus. Further molecular identification with 16S-rRNA analysis revealed that HF.7 isolate had 99% similarity with Burkholderia lata.

Keywords

Endophytic bacteria, Phytase, Phytic Acid, Zea mays

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