ISSN: 0973-7510

E-ISSN: 2581-690X

Neda Sadat Khademestarki1 and Reza Ranjbar2
1Department of Microbiology, Islamic Azad University, Damghan Branch, Damghan, Iran.
2Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
J. Pure Appl. Microbiol., 2016, 10 (1): 351-354
© The Author(s). 2016
Received: 13/06/2015 | Accepted: 19/09/2015 | Published: 31/03/2016
Abstract

Urinary tract infection (UTI) is one of the common infectious diseases.  Escherichia coli is one of the most important cause of UTI. The purpose of this research was to determine the frequency of some phylogenetic genes in E. coli strains isolated from patients with UTI. E. coli strains were recovered from urine culture of suspect patients with UTI and biochemical and bacterial tests have been utilized to identify the isolated bacteria. Detection of  phylogenetic genes were done through Multiplex PCR. Approximately 94%, 92%, 82% and 72% of E. coli strains contained ChuA1, YjaA , TspE4C and arp genes respectively. Results indicate that PCR based phylogenetic molecular method is a simple and quick method for typing of our uropathogenic E. coli.

Keywords

Uropathogenic E. coli, Phylogenic, PCR.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.