With the development of network theory and the metabolic network research tools, metabolic network has been gradually used to guide biomass production. This study analyzed the topology and interaction of the metabolic network of Bacillus subtilis based on computer simulation environment through Constraints Based Reconstruction and Analysis(COBRA) toolbox. By removing several reactions part and analyzing the flux distribution in FBA and FVA, we illustrate the correlation between one single reaction and system. We further verify the feasibility and applicability of the network theory and model method between reactions, so as to understand the topological and functional characteristics of Bacillus subtilis metabolism network.