ISSN: 0973-7510

E-ISSN: 2581-690X

Yongjun Dong, Lirong Wang , Xingyou Liu and Kun Zhao
1College of Animal Science, Henan Institute of Science and Technology, Xinxiang, Henan, 453003, China.
J Pure Appl Microbiol. 2015;9(2):899-911
© The Author(s). 2015
Received: 25/12/2014 | Accepted: 01/02/2015 | Published: 30/06/2015
Abstract

Seven strains of bacteria were isolated from clinical cases of swine disease in lungs and trachea. The isolated bacteria was identified as Actinobacillus pleuropneumoniae (APP) serotypes 1 through bacterial cultivated, culture characteristic observation, morphology, biochemical, growth of satellite phenomenon, hemolysis and serotype identification. The antimicrobial susceptibility showed that the isolated A. pleuropneumoniae resistance to penicillins, semisynthetic antibiotics, b-lactam antibiotics, polypeptide antibiotic, polyene antibiotic, coumarin antibiotic, amphenicols antibiotics, b-lactam/b-lactam inhibitor compounds, cephalosporins, aminoglycosides, tetracyclines, fluoroquinolones, macrolides, sulfa drugs, sugar peptide drugs, furan drugs, ethlhydrocupreine; medium sensitive to cephalothin (CF), cefizime (CEX), ciprofloxacin (CIP), norfloxacin (NOR), ofloxacin (OFL); high sensitive to polymyxin B (PB), fosfomycin (FOS), ceftizoxime (CTZ), cefmetazole (CMZ), cefaclor (CEC), cefoperazone/sulbactam (CFP/SU), cefetamet (CTM), cefotaxime (CTX), ceftazidime (CAZ), cefodizine (CDZ), cefurosimc sodium (CXM), cefotaxime/clayulanic acid (CTX/CA), ceftazidime/clavulanic acid (CAZ/CA), cefoxitin (FOX), cefepime (FEP), ceftriazone (CRO),aztreonam (AZT), minocycline. This result showed that A. pleuropneumoniae clinical strains had multi-drug resistance and resistance characteristics, which means that these isolated bacteria was multiple drug resistant strains.

Keywords

Actinobacillus pleuropneumoniae, Isolation, Identification, Serotype, Antimicrobial resistance, antimicrobial susceptibiligy, Multidrug resistance

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