ISSN: 0973-7510

E-ISSN: 2581-690X

Xiaodan Qi1, Haitao Yu2, Yan Sun3, Hao Cheng4 and Chunjing Zhang5
1Department of Clinical Biochemistry, Qiqihar Medical University, Qiqihar, China.
2Department of Biology Genetics, Qiqihar Medical University, Qiqihar, China.
3Department of Clinical Pathogenic Microorganism, Qiqihar Medical University, Qiqihar, China.
4The Clinical College Students, Qiqihar Medical University, Qiqihar, China.
5Department of Biochemistry and Molecular Biology, Qiqihar Medical University, Qiqihar, China.
J Pure Appl Microbiol. 2013;7(2):1317-1323
© The Author(s). 2013
Received: 07/04/2013 | Accepted: 28/05/2013 | Published: 30/06/2013
Abstract

Influenza A virus targets the lung epithelial cells for infection and produces clinical outcomes ranging from a mild upper respiratory infection to severe pneumonia. Baicalin is Chinese herbal monomer of phenolic hydroxyl flavonoids extracted from scutellaria baicalensis of heat-clearing and detoxicating drug, has better anti-inflammatory function, antioxidant function and antiviral activity, but the mechanism of the anti-influenza viral activity of baicalin has not been revealed. The research object of the study is to discuss the significant activity and part mechanism of baicalin against influenza A viruses. Antiviral efficiency of baicalin in vitro was observed with trypan blue staining method. RT-PCR was used to study the impact of baicalin on influenza A virus structural protein HA, NA, M gene replication. Intracellular generation of reactive oxygen species (ROS) was examined by flow cytometry using probe DCFH-DA. Antioxidant reagent kits were applied to detect Superoxide dismutase (SOD) activity, malondialdehyde (MDA) content. The results showed that baicalin has a better protective effect on cell damage from influenza A virus. Compared to virus infected group, HA, NA, M gene replication in high concentration baicalin (50µg/ml) treatment group was in a decreasing trend respectively, but there has no significant difference. Baicalin can reduce influenza virus-induced ROS production in a dose-dependent manner, and decrease the production of malondialdehyde (MDA)ÿthe end product of lipid peroxidationÿimprove the activity of antioxidant enzyme SOD, and so reduce influenza virus infection-induced oxidative-stress damage.

Keywords

Baicalin, influenza virus, HA, NA, M gene replication, oxidative stress

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