ISSN: 0973-7510

E-ISSN: 2581-690X

Sheli Chai1 , Jia Guo1, Yuan Chai2, Jing Cai1 and Qingwei Zhang3
1College of Geo-exploration Science and Technology,Jilin University, Changchun – 130 026, China.
2College of Geosciences,Jilin University, Changchun – 130 061, China.
3College of Earth Sciences,Quilin University of Technology, Guilin – 541 004, China.
J Pure Appl Microbiol. 2013;7(Spl. Edn.: November):753-758
© The Author(s). 2013
Received: 27/09/2013 | Accepted: 04/11/2013 | Published: 30/11/2013

The effect of trace element addition on the anaerobic digestion of wine vinasse in an anaerobic moving bed biofilm reactor was investigated in terms of applied organic loading rate, soluble COD removal efficiency, effluent propionate content and biogas production rate during the whole experiment. The addition of the first series of trace element combination with Fe (50 mg/l), Ni (10 mg/l), Co (10 mg/l) from day 178 to 181 did significantly optimize the reactor performance although the propionate levels were still high with ranges from 1.01 to 3.34 mg/l. Associated to this addition, the well-buffered pH system of the reactor was established, which made the reactor operated under the acidic influent. The addition of the second series with individual Fe from day 210 to 213 and the addition of the third series of trace element combination with Fe and Ni from day 214 to 217 did not affect the performance of the reactor distinctly. The addition of the fourth series of trace element combination with Fe (50 mg/l), Ni (10 mg/l), Co (10-20 mg/l), Cu (2 mg/l), Zn (20 mg/l), and Mo (0.8 mg/l) from day 218 to 225 can markedly reduce the propionate levels from about 2.0 g/l to 0.5 g/l, resulting in the performance of the reactor to be enhanced greatly. At the end of the experiment, soluble COD removal efficiency of the reactor was up to about 86% with an organic loading rate of 18.43 g COD/ l d, effluent propionate level was below 1.0 g/l, and biogas production rate was 8.6 l/l d. The addition of trace elements to the influent of the reactor resulted in a steady accumulation of Ni, Co and Mo in the suspended and attached biomass. The experiment result showed that using trace element combination to stimulate the performance of the reactor was quite available with the short-term dosing strategy, providing a promising method for sustainable anaerobic digestion of high strength wastewater.


Trace element, Anaerobic degradation, Wine vinasse, Biomass, Anaerobic moving bed biofilm reactor

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