In order to evaluate the effect of cytokinin and iron on biological nitrogen fixation in chickpea cultivars, experiment was conducted as a factorial experiment in randomized complete block design in four replications. The treatments consisted of three cultivars: (Hashem, Azad and local mass),four levels of iron [control (lack of consumption), 5, 10 and 15 ppm] and four levels of cytokinin (6-Benzyl aminopurin) [control (lack of consumption), 5, 10 and 15 ppm]. Local mass with 3.69, 6.1 and 3.78 percent, had the highest nitrogen percent in the above ground, noude and grain. By increasing concentrations of iron, increased nitrogen percent in aboveground and grain, but in noude  increased up to 10 ppm and then decreased. However, by increasing concentrations of cytokinin , the nitrogen percent of grain increased, but nitrogen percent of aboveground and noude  increased up to 10 ppm and then decreased. Local mass with 129.8 kg/ha had the highest nitrogen fixation. Effects of iron and cytokinin on nitrogen fixation was positive, and by increase their concentration, nitrogen fixation increased and in concentration of 15 ppm content of fixed nitrogen were 130.9 and 129.8kg/ha respectively. From of all fixed nitrogen in plant, Contribution of aboveground, grain and noude were 46.19, 46.41, and 7.4 percent.