ISSN: 0973-7510

E-ISSN: 2581-690X

Kianoush Hamidian1 , Ahmad Naderi2*, Shahram Lak3 and Islam Majidi4
1Department of agronomy, Callege of Agriculture, Khuzestan Science and research Branch, Islamic Azad University, Ahvaz, Iran.
2Faculty of Agriculture and Natural Resource Research Center of Khuzestan, Ahvaz, Iran.
3Department of agronomy, Callege of Agriculture, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran.
4Department of Plant Breeding, Callege of Agriculture,Tehran Science and Research Branch, Islamic Azad University, Tehran, Iran.
J. Pure Appl. Microbiol., 2016, 10 (1): 383-388
© The Author(s). 2016
Received: 02/09/2015 | Accepted: 17/01/2016 | Published: 31/03/2016

In order to evaluate the effect of cytokinin and iron on biological nitrogen fixation in chickpea cultivars, experiment was conducted as a factorial experiment in randomized complete block design in four replications. The treatments consisted of three cultivars: (Hashem, Azad and local mass),four levels of iron [control (lack of consumption), 5, 10 and 15 ppm] and four levels of cytokinin (6-Benzyl aminopurin) [control (lack of consumption), 5, 10 and 15 ppm]. Local mass with 3.69, 6.1 and 3.78 percent, had the highest nitrogen percent in the above ground, noude and grain. By increasing concentrations of iron, increased nitrogen percent in aboveground and grain, but in noude  increased up to 10 ppm and then decreased. However, by increasing concentrations of cytokinin , the nitrogen percent of grain increased, but nitrogen percent of aboveground and noude  increased up to 10 ppm and then decreased. Local mass with 129.8 kg/ha had the highest nitrogen fixation. Effects of iron and cytokinin on nitrogen fixation was positive, and by increase their concentration, nitrogen fixation increased and in concentration of 15 ppm content of fixed nitrogen were 130.9 and 129.8kg/ha respectively. From of all fixed nitrogen in plant, Contribution of aboveground, grain and noude were 46.19, 46.41, and 7.4 percent.


Chickpea, Biological nitrogen fixation, Cytokinin, iron, Aboveground, Noude and grain.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.