Cytological study is an important basis to analyze tomato grafting body healing. However, sample fixed is a key step to begin cytology study. Fixative type, concentration, fixed time and its temperature will be affected by the result of tissue fixation, so further influence the subsequent experiments. This paper observes different fixatives on tomato stem cells and cell inclusions of fixed effects using a simple\direct hand sectioning with spontaneous fluorescence. The fixed effect of 2.5% glutaraldehyde fixation fluid of tomato stem is the best method in the three that the cellular structure of all tissues remained intact under light microscopic and complete chloroplast aglow phenomenon, a spontaneous fluorescence, can be observe in cells. The fixed effect of 50% alcohol fixed fluid to stem is poorer, and there is no cell fluorescence phenomenon through spontaneous fluorescence, and the autofluorescence of cuticular layer and epidermal hair also decreased significantly. The chloroplasts in cells can be fixed by the FAA fixed liquid, and through the cuticular layer and epidermal hair spontaneous fluorescence observation were not weakened, but the fixed effects of cell wall are not better compared with glutaraldehyde fixation fluid. When fixed plant stem, therefore, consider using 2.5% glutaraldehyde fixation fluid is very necessary.
Tomato, Fixative, Spontaneous fluorescence
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