Inflammatory mediators produced by fibroblasts as the main reagents of wound healing have fundamental roles in this process. Due to stimulatory effects of lipopolysaccharide (LPS) on different biological mechanisms of mammalian cells and its influence on pro-inflammatory cytokines, this bacterial endotoxin has been surge of interest in immunological and inflammatory studies. Skin is the first barrier in immune response which is more susceptible to inflammation. In this investigation, the effects of Salmonella enterica LPS on skin fibroblast cells viability was evaluated. Nitric oxide (NO), cyclooxygenase-2 (COX-2) and hydrogen peroxide (H₂O₂) levels were assessed after LPS treatment of fibroblasts. Human foreskin fibroblasts were treated by different concentrations of Salmonella enterica LPS (100µg~0.01µg). Effects of LPS on cell viability and NO, COX-2 and H₂O₂ levels were examined respectively by XTT assay and related kits as per the manufacturer’s protocols. Results of present survey illustrate that there is a dose and time dependent significant difference between control and treated cells in cell proliferation. Results obtained from assays indicate that LPS stimulates NO, COX-2 levels and reduces H₂O₂ levels in fibroblast cells (p-value<0.001). According to LPS effects on cell proliferation in dose and time dependent manner and increasing nitric oxide as a vital factor in the healing process, it will be considered theraputical potential of this bacterial endotoxin.