This paper is mainly to discuss the drug resistance of pseudomonas aeruginosa and control measures of hospital infection, which will provide scientific basis for effective control of infection. The identification is performed with automatic bacteria analyzer and drug susceptibility system, and 13 kinds of antimicrobial drug susceptibility tests are carried on 485 strains of pseudomonas aeruginosa .The drug resistance of pseudomonas aeruginosa on cefazolin is as high as 100%, cefotaxime and cefoperazone also shows high drug resistance, with its rate reaching as high as 58.9% and 51.6% , followed by gentamicin , oxygen fluorine oxazine penicillin and aztreonam, whose rate of resistance is 31.3%, 32.9% and 31.3% respectively. With a large number of broad-spectrum antibiotic commonly used, the drug resistance of antibiotic commonly used in pseudomonas aeruginosa is on the rise in general , which also has emerged the trend of multiple drug resistance. Therefore the hospital should make reasonable plan to fight infection, put forward practical and effective management measures to control patients with abuse situation of antibacterial drug through study.