Probiotic bacteria with an ability to change micro flora of intestine play an important role in body’s health. Probiotics are also suitable alternatives for antibiotics. The objective of this study is to obtain the indigenous Bacillus strains isolated from poultry farms of Arak city of Iran and to evaluate their probiotic properties. A total number of 41 samples of fresh fecal matters were collected from 8 poultry farms of Arak city. Probiotic properties of the strains were then determined (as for their resistance against acid, bile, pepsin, gizzard extract and production of antimicrobial compounds). In this study, a total number of 140 Bacillus isolates were screened of which 14 isolates were utilized for the purpose of preventing hemolysis in the forthcoming studies. Study of probiotic properties showed that strain 8 was resistant at 100% against Hydrochloric acid and strain 5 was resistant at 100% against bile salts. More than 80% of the selected strains were resistant to gizzard extract and pepsin. All strains possessed an antimicrobial activity against the common poultry pathogens. This study exhibited that the selected bacillary isolates possessed probiotic properties and they could be used as a poultry probiotic after thorough examinations and field tests.