ISSN: 0973-7510

E-ISSN: 2581-690X

S. Nandwani , M. Choudhry, S. Misra and S. Gupta
Department of Microbiology, ESIC- PGIMSR & Hospital, Basaidarapur, New Delhi – 110 015, India.
J Pure Appl Microbiol. 2012;6(Spl. Edn.: October):111-115
© The Author(s). 2012
Received: 04/03/2012 | Accepted: 10/06/2012 | Published: 31/10/2012
Abstract

Hepatitis B viral (HBV) infection is a leading cause of liver disease worldwide.  This study was done to see the prevalence of hepatitis B in patients reporting at our hospital and evaluation of two rapid Immunochromatographic testing (ICT) kits for HBs Ag detection. A total no. of 5024 serum samples were collected wef Jan 2011 to July 2011 from patients and were tested for HBs Ag by ELISA. The two ICT tests- InstachkTM by InTec Products INC and HEPATMCARD by Reckon Diagnostcs Pvt. Lt. were performed on Elisa tested samples for HBV- Reactive (n = 137) and HBV- Non reactive (n = 150) samples. Prevalence of Hepatitis B in our study was found to be 2.73 %, 137 being reactive out of 5024 samples by ELISA. The I.C.T. results showed a sensitivity of 94.16 % and 51.09%, specificity of 100% and 96.67 %, positive predictive values of 100% and 93.33 % while the negative predictive values of 94.93 % and 68.39 % for InstachkTM and HEPATMCARD kits respectively. These results suggest that the rapid ICT kits for HBsAg have only limited efficacy and should be backed by superior methods like ELISA and PCR where possible.

Keywords

Prevalence, HBV, hepatitis, ELISA, Immunochromatographic tests, HBs Ag

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© The Author(s) 2012. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.