Three strains, 13#, 15# and 17#, which had ability of denitrifying phosphorus-uptake, were isolated and identified from a low carbon-source treatment system. Through 16S rDNA sequence analysis and homology comparison, three strains were identified as Klebsiella pneumonia strain, Ralstonia pickettii strain and Acinetobacter junii strain, respectively. The nitrogen and phosphorus removal study showed that all three strains are denitrifying phosphorus removal bacteria, especially the 13# which is considered as the efficient DPAOs for treating low carbon source sewage. The phosphorus release and phosphorus uptake amounts of 13# were 0.72 and 0.88 mg/L, respectively. 15# and 17# did not absorb excess phosphorus because of the high NO₃^–-N concentration (>50mg/L). Furthermore, 13# and 15# are capable of denitrification under aerobic condition suggesting that the LS-SDP existed simultaneous nitrification and denitrification (SND) besides denitrifying phosphorus removal.