ISSN: 0973-7510

E-ISSN: 2581-690X

CH. Mina Kumari, I.Bhaskar Reddy and K. Vijaya Rachel
Department of Biochemistry, GIS, GITAM University, India.
J Pure Appl Microbiol. 2010;4(2):823-826
© The Author(s). 2010
Received: 30/03/2010 | Accepted: 08/05/2010| Published: 31/10/2010
Abstract

The present investigation deals with the screening and study on the amylolytic activity of bacteria isolated from flour mill waste. Six different bacterial colonies were isolated and tested for starch hydrolysis. Out of them, isolate no A4 has showed maximum zone of hydrolysis of 3.3 mm in starch agar medium and identified as Bacillus. The isolated Bacillus was subjected for amylase assay and extra cellular amylase production. The maximum amylase production was achieved with a reducing sugar concentration of 2.25 IU/ml at 37 0C for 48 hours of incubation.

Keywords

Bacillus, amylase, starch hydrolysis, extra cellular amylase

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