Study on Endotoxin Removal from Human-like Collagen

ISSN: 0973-7510

E-ISSN: 2581-690X

Huizhi Zhang¹, Daidi Fan¹ , Jianjun Deng¹, Xiaoxuan Ma¹, Chenghui Zhu¹ and Wenjiao Xue²

¹Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical
Engineering, Northwest University, Xi’an, Shaanxi, China.

²Shaanxi Provincial Institute of Microbiology, No. 76 Xiying Road, Xi’an – 710 043, China.

J Pure Appl Microbiol. 2013;7(1):435-439

Received: 11/01/2013 | Accepted: 27/02/2013 | Published: 31/03/2013

Abstract

In this study endotoxin is removed from protein preparations using a Pierce^® High-Capacity Endotoxin Removal Resin column. The effects of phosphate buffer and NaCl concentration on endotoxin removal and protein recovery have been investigated. Both PBS concentration and NaCl had significant effects on endotoxin removal from protein solutions. When PBS and NaCl were increased from 10 to 40mM and from 100 to 200mM respectively, the residual endotoxin was reduced from 8000 to 25 EU/ml in the PBS 25mM and NaCl 150mM. When protein concentration was varied from 1 to 5mg/ml, the residual endotoxin was reduced to about 20EU/ml in the PBS 25mM and NaCl 150mM. However, the incubation time had a critical effect on the endotoxin removal. Protein recovery was above 98% in all cases. This method greatly provided high endotoxin removal and HLC recovery.

Keywords

Endotoxin removal, Human-like Collagen, Endotoxin-protein Complex, Lipopolysaccharides

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