ISSN: 0973-7510

E-ISSN: 2581-690X

Suvarna Joshi1, Yagnesh Thakar2 and Ashok Gomashe1
1Shri Shivaji Science College, Congress nagar, Nagpur, India.
2Vishakha Clinical Microbiology Laboratory, 612, A-wing, Lokmat Bhawan, Wardha Road, Nagpur – 440 012, India.
J Pure Appl Microbiol. 2009;3(2):679-683
© The Author(s). 2009
Received: 05/03/2009 | Accepted: 18/04/2009| Published: 31/10/2009
Abstract

A total of 40 urinary isolates of E.coli from patients of U.T.I. and 30 faecal isolates from healthy persons were studied for O serotyping; Haemolysin production; Haemagglutination; Congo red binding. Haemolysin was positive in 21 (52.5%) of urinary isolates as against only 9 (30%) of faecal isolates; Haemagglutination was positive in 23 (57.5 %) of which 18(45%) were mannose resistant Haemagglutinating while only 10 (33.3%) of faecal isolates were Haemagglutinating. All the 40 (100%) Urinary isolates and as many as 28(93.3%) out of 30 faecal E.coli were able to bind Congo red dye. O serotyping was possible in 65% of uropathogenic and 63.3% faecal E.coli strains. The early serogroups viz; O2; O4; O8; O23; O25 were commonest. Urinary isolates of E.coli were sensitive most to Nitrofurantoin (97.5%), followed by Amikacin (95%), Gentamicin (82.5%), Ceftazidime (82.5%) and Amoxycillin+Clavulanic acid (75%), while the sensitivity was even less than 50% to Cephotaxime (47.5%), Ceftriaxone (45%),  Cefuroxime (37.5%), Cefixime (25%), Ciprofloxacin (22.5%), Cotrimoxazole (17.5 %), Norfloxacin (17.5%), Ampicillin (12.5%). Thus study shows haemolysin and haemagglutination are reliable markers of virulence in uropathogenic E.coli. Congo red binding is not good marker and there is prevalence of limited serogroups of uropathogenic E.coli in this region and the isolates are generally multidrug resistant.

Keywords

Uropathogenic E. coli, Urinary tract infections, Haemagglutination, Haemolysin, Congo red binding

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© The Author(s) 2009. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.