ISSN: 0973-7510

E-ISSN: 2581-690X

Vishal Kumar Deshwal1 , Shipra Agarwal2 and Zubair Ahmad3
1Department of Microbiology, Doon (PG) Paramedical College, Dehradun, India.
2Department of Biotechnology, Institute of Foreign Trade & Management (IFTM), Moradabad, India.
3Department of Microbiology, Teerthankar Mahaveer Dental College & Research Centre,
(TMU), Moradabad, India.
J Pure Appl Microbiol. 2011;5(1):453-456
© The Author(s). 2011
Received: 29/08/2010 | Accepted: 21/10/2010 | Published: 30/04/2011

Coagulase Negative Staphylococcus (CNS) strains were isolated from hospital personnel and hospital environment. Total 320 samples were taken from skin, anterior nares, nails, finger tips, stethoscope, floor, door handle and catheter. Samples were spread on Mannitol Salt agar Medium and one Staphylococcus culture from each sample was select for further study. Isolated CNS strains were characterized according to Bergey’s manual of determinative bacteriology. Results suggested that five types of CNS strains- Staphylococcus saprophyticus, Staphylococcus epidermidis, Staphylococcus hominis, Staphylococcus haemolyticus, Staphylococcus sp. were isolated from hospital personnel and hospital environment and 24.68% strains were Coagulase negative staphylococcus (CNS). Maximum (CNS) strains were present in anterior nares and it was 42.5%. CNS was also present in skin (22.5%), nails (30%), finger tip (27.5), stethoscope (27.5), floor (15%), door handle (20%) and catheter (12.5).


CNS, S. saprophyticus, S. epidermidis, S. hominis, S. haemolyticus

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© The Author(s) 2011. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.