Coagulase Negative Staphylococcus (CNS) strains were isolated from hospital personnel and hospital environment. Total 320 samples were taken from skin, anterior nares, nails, finger tips, stethoscope, floor, door handle and catheter. Samples were spread on Mannitol Salt agar Medium and one Staphylococcus culture from each sample was select for further study. Isolated CNS strains were characterized according to Bergey’s manual of determinative bacteriology. Results suggested that five types of CNS strains- Staphylococcus saprophyticus, Staphylococcus epidermidis, Staphylococcus hominis, Staphylococcus haemolyticus, Staphylococcus sp. were isolated from hospital personnel and hospital environment and 24.68% strains were Coagulase negative staphylococcus (CNS). Maximum (CNS) strains were present in anterior nares and it was 42.5%. CNS was also present in skin (22.5%), nails (30%), finger tip (27.5), stethoscope (27.5), floor (15%), door handle (20%) and catheter (12.5).
CNS, S. saprophyticus, S. epidermidis, S. hominis, S. haemolyticus
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