ISSN: 0973-7510

E-ISSN: 2581-690X

Hao Huang , Zhonghua Yang, Guanghui Wang and Jun Chen
1Biological Engineering Institute, Chemical Engineering and Technology College, Wu Han University of Science and Technology, Wu Han, China.
J Pure Appl Microbiol. 2013;7(Spl. Edn.: November):711-717
© The Author(s). 2013
Received: 27/09/2013 | Accepted: 04/11/2013 | Published: 30/11/2013

Oligo-glucomannan (OGM) could be obtained through enzymolysis from KGM, a water-soluble polysaccharide isolated from Konjac flour. The sulfated derivative of OGM (OGMS) was prepared by chlorosulfonic acid method with formamide as a dehydration-condensation agent. OGMS was one kind of low molecular weight polysaccharide sulfate which had different weight-average molecular mass (Mw) ranging from 1.8 to 2.0 KDa and different degree of substitution (DS) ranging from 1.25 to 1.73. FT-IR and 13C NMR spectra indicated that the sulfated groups had been introduced at C-2 0C-6 and C-3 positions of OGMS. OGMS had significant inhibitory effect on the growth of HepG2 and Hela cells in vitro at concentration higher than 0.2mg/mL. On the other hand, OGMS was a potent inhibitor of viruses HBV, IAV and CBV3, with IC50 values determined in vitro of 8.3, 4.2 and 3.1µÀgmL-1, respectively due to its sulfated groups and low molecular weight. The results showed that konjac oligo-glucomannan sulfate could be developed as a new type of medicine which had potential antiviral activity.


Sulfated derivative, Oligo-glucomannan, Cytotoxicity, Antiviral

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