ISSN: 0973-7510

E-ISSN: 2581-690X

M. Radhakrishnan1, R. Pazhanimurugan2, V. Gopikrishnan2, Vanaja Kumar1 and R. Balagurunathan2
1Centre for Drug Discovery and Development, Sathyabama University, Chennai – 600 119, India.
2Department of Microbiology, Periyar University, Salem – 600 011, India.
J Pure Appl Microbiol. 2014;8(1):333-337
© The Author(s). 2014
Received: 12/09/2013 | Accepted: 29/10/2013 | Published: 28/02/2014

While screening actinomycete isolates for anti-TB natural products actinomycete strain D25, isolated from Thar Desert soil, Rajasthan, was observed to produce yellow-orange pigmented anti-TB compound in solid culture. The effect of medium co and nutrient concentration on the growth and anti-TB pigment production by the strain D25 was studied. Further, the effect of mechanical shaking and that of the producing organism itself on the yellow orange pigment production was studied by submerged fermentation. Strain D25 was found to produce the bioactive yellow-orange pigment only on solid culture but not in liquid culture in which the pigment got degraded either by mechanical shaking or by the enzyme action of the producing strain D25 itself. Effect of medium consistency and nutrient concentration was studied by using different concentrations of YEME broth and YEME agar. Medium consistency and filamentous mycelial structure did not have significant effect on bioactive pigment production by the strain D25 even while using different nutrient concentrations.


Actinomycetes, Streptomyces, Thar Desert, solid culture, Antituberculous

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