ISSN: 0973-7510

E-ISSN: 2581-690X

Mengwei Xiao1, Kaiyu Wang1,2 , Defang Chen3, Jun Wang1, Lanmin Li1, Xingxing Liu1, Yi Geng1,2, Xiaoli Huang3 and Dan Xiao4

1Department of Basic Veterinary, Veterinary Medicine College, Sichuan Agricultural University, Ya’an 625014, Sichuan, P.R. China.
2Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Ya’an 625014, Sichuan, P.R. China.
3Department of Aquaculture, College of Animal Sicence& Technology, Sichuan Agricultural University, Ya’an, Sichuan 625014, P.R. China.
4Animal Health Research Institute of Tongwei Co., Ltd., Chengdu, Sichuan 610041, PR China.
J. Pure Appl. Microbiol. 2014, 8(6):4297-4307
© The Author(s). 2014
Received: 06/07/2014 | Accepted: 04/09/2014 | Published: 31/12/2014
Abstract

Specific primers were used to amplify the gene cfi encoding CAMP factor of Streptococcus iniae (S. iniae) DGX07. CAMP factor of natural form of S. iniae could be detected by Western-blot with anti-recombinant CAMP factor antiserum. Western-blot suggested that the amount of CAMP factor expressed in S. iniae were very low and that might be not virulent enough to trigger immune response in rabbits. Western-blot also demonstrated its non-specific binding ability to rabbit immunoglobulin G.CAMP factor showed binding and invasion ability to Epithelioma papulosum cyprini cells in vitro. However, hemolysis showed the recombinant CAMP factor could exhibit the classic co-hemolytic activity on some mammals’red blood cells but failed to cytolyse fish red blood cells, suggesting that CAMP factor lost its co-hemolysis to fish in S.iniae.

Keywords

cfi gene, molecular analysis, co-hemolysis, cell adherence and invasion, IgG binding

Article Metrics

Article View: 0

Share This Article

© The Author(s) 2014. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.