ISSN: 0973-7510

E-ISSN: 3179-3185

Lakshminarayanan Vijayakumar1 , Ponnuswamy Ramalingam2, Sundaramurthi Pavithra1 and Rengaraju Balakrishnaraja1
1Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam – 638401, Tamil Nadu, India.
2Department of Biotechnology, Kumaraguru College of Technology, Coimbatore – 641006, Tamil Nadu, India .
© The Author(s). 2015
J. Pure Appl. Microbiol., 2015, 9 (4): 3179-3185.
Received: 18/10/2015 | Accepted: 06/12/2015 | Published: 31/12/2015
Abstract

β β-1,3 glucan, an Exopolysaccharide (EPS) that has enormous industrial applications can be produced commercially through microbial fermentations. The huge market demand necessitates the industries to search for new sources or to implement new strategies to maximize the yield of glucan. Hence, the present study focused on optimization of components of the culture medium and process variables to increase â1,3 glucan production from Bacillus cereus LVK13 (KC 898956) using statistical tools. The essential nutrients that influence â-glucan production was screened using PlackettBurman Design (PBD). Further, the three-screened components out of the eleven were analyzed with central composite design (CCD). The optimum concentrations of carbon, nitrogen and manganese sources were found to be 80, 1.5 and 0.03 g/l. Second order polynomial regression model accurately showed interpretation of experimental data with an R2 value of 0.9563, Adjusted (Adj) R2, Predicted (Pred) R2 and F values of 0.9474, 0.9329 and 182.69 respectively.

Keywords

â-glucan, Sucrose, Bacillus cereus LVK13, Optimization, RSM.

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© The Author(s) 2015. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.