ISSN: 0973-7510

E-ISSN: 2581-690X

B.V. Gondhali , T.A. More, S.S.Pawar, S.A. Ranpise and V.G. Khandagle
Department of Horticulture, Mahatma Phule Krishi Vidyapeeth, Dist. Ahmednagar, Rahuri – 413 722, Maharashtra, India
J. Pure Appl. Microbiol., 2016, 10 (2): 1395-1401
© The Author(s). 2016
Received: 03/12/2015 | Accepted: 15/01/2016 | Published: 30/06/2016
Abstract

In the present study, MS media was found to be the best media among the three different media tried for the establishment of explants. Among the different periods of surface sterilization with 0.1 % HgCl2, 3 min for shoot tip and 4 min for nodal segment were found to be appropriate for surface sterilization which recorded 85.50 per cent  in vitro culture survival in shoot tips and 89.25 per cent in vitro culture survival in nodal segment. The significantly maximum establishment for shoot tips (12.00 %) and nodal segment (66.67%) was recorded on MS medium. MS media supplemented with 2.0 mg/l BAP and 20 mg/l AS was found suitable for maximum establishment in shoot tips (10.67 %) and nodal segments (66.67%). MS media supplemented with 2.5 mg/l BAP + 0.5 mg/l NAA + 2.5 g/l AC was found to be most efficient media for multiplication through nodal explants which produced more number of shoots per explant (11.30) with 62.53 shooting per cent. The ½ MS media supplemented with 1.5 mg/l IBA + 2.0 g/l AC was most suitable for rooting which recorded maximum number of roots/shoot (4.50), more length of roots (3.53 cm), maximum  rooting percentage (40.63) and maximum survival of tissue culture plants (82.03 %).

Keywords

Pomegranate, In vitro regeneration, Establishment, Multiplication, Rooting.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.