Papilloma viruses are highly species specific, infecting Humans only. More than 70 types of HPV have been identified, some of which are thought to cause cancerous and pre-cancerous conditions of the cervix. In many countries, HPV is the most common sexually transmitted infection (STI).  In women worldwide, cervical cancer remains the second most common cancer and that affects women mostly. In countries where screening is not implemented, cervical cancer is still a major health problem and a frequent cause of death. Cytology screening (e.g. Pap test) is the standard method used for the control of cervical cancer in India. Despite the availability of Pap testing even on an opportunistic basis in India, the incidence of invasive cervical cancer remains high, especially in rural India. Pap smear and DNA detection by PCR are test done to detect HPV infection.  Out of 11 samples processed 4 were found to be HPV Positive and remaining 7 were negative which were in correlation with the Histopathological examination. PCR can help in early detection of HPV DNA; this can help in diagnosis and further treatment for HPV.