ISSN: 0973-7510

E-ISSN: 2581-690X

F. Badillo1, A. Rivera1,2 , O. Romero1,3, A. Aragón1,3, M. Huerta4, R. Santellan2 and E. Reyes5
1Postgraduate Degree in Sustainable Agroecosystem Management, Autonomous University of Puebla, Mexico.
2Center for Research in Microbiological Sciences, Autonomous University of Puebla.
3Center for Agroecology, Autonomous University of Puebla, Mexico.
4University Department of Sustainable Development, Autonomous University of Puebla, Mexico.
5Applied Research Laboratory of the University Center for Bonding, Autonomous University of Puebla, Mexico.
J Pure Appl Microbiol. 2014;8(5):3957-3962
© The Author(s). 2014
Received: 29/05/2014 | Accepted: 16/07/2014 | Published: 31/10/2014
Abstract

Mollicutes are the smallest organisms capable of autonomous self-replication, and their phenotype differs from that of other bacteria in their lack of a cell wall. Mollicutes are wide spread in nature and pathogenically infect humans, other mammals, reptiles, arthropods and plants. Normally demonstrate a strict specificity to a host, as well as tissue specificity, which result from an obligate parasitic lifestyle. However, plant pathogens, including phytoplasmas, spiroplasmas and acholeplasmas, have the capability to infect a variety of plants and use insect-host vectors for transmission. The objective was detection and characterization of spiroplasmas by PCR, sequencing 16S rRNA, and atomic force microscopy, in isolates of Apis mellifera in Puebla-Mexico. Samples from 100 honey bee were collected and examined by PCR, sequencing 16S rRNA and atomic force microscopy. A 271 bp product was produced from the F28/R5 primer set in 38%. Alignment of the sequences showed a 95% similarity with Spiroplasma melliferum. Atomic force microscopy study shows the presence of spherical structures formed chains with sizes within the ranges reported for spiroplasmas.

Keywords

Apis mellifera, spiroplasma, vector, PCR, AFM

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© The Author(s) 2014. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.