ISSN: 0973-7510

E-ISSN: 2581-690X

I.D. Anand1 , M.R. Ravikumar2, S. Raghu3, H. Virupaksha Prabhu1 and M. Ranganath Swamy1
1Department of Plant Pathology College of Agriculture, Dharwad, India.
2Department of Plant Pathology College of Agriculture, Hanumanamatti, University of Agricultural Sciences, Dharwad – 580 005, Karnataka, India.
3Crop protection division ICAR-Central Rice Research Institute, Cuttack – 753 006, Odisha, India.
J Pure Appl Microbiol. 2015;9(Spl. Edn. 2):259-264
© The Author(s). 2015
Received: 11/04/2015 | Accepted: 26/06/2015 | Published: 30/11/2015
Abstract

The wilt caused by bacteria (Ralstonia solanacearum) is the most devastating pathogen among all the rhizome rot pathogens. An effort was made for quick detection of bacterial wilt pathogen using PCR based molecular techniques. Phylogram results of four RS isolates Revealed that there are two major clusters, cluster A comprising all Ralstonia solanacearum species, A glance towards dendrogram reveals that there was not much diversity among the four isolates of RS. The  fliC  gene  was  amplified  and an  expected  size of 400bp it was confirmed the Ralstonia solanacearum flagellin protein (fliC) gene. Fragment of fliC gene sequences of RS-1 (Haveri) and RS-8 (Shimoga) isolates showed 99 per cent similarity with NCBI published sequence of RS Strain MR11 plasmid mega plasmid flagellin protein (fliC) gene, partial cds (KF031064.1) and DSM9544 flagellin (fliC) gene, partial cds (AY192724.1) respectively. Dharwad isolate showed 98 per cent similarity with Ralstonia solanacearum strain RCR-226 flagellin protein (fliC) gene, partial cds (KC834785.1). While Uttara Kannada isolate showe 97 per cent similarity with RS flagellin (fliC) gene, complete cds (AF283285.1).

Keywords

Ralstonia solanacearum, Bacterial wilt, quick detection, fliC gene, Polymerase chain reaction

Article Metrics

Article View: 896

Share This Article

© The Author(s) 2015. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.