The wilt caused by bacteria (Ralstonia solanacearum) is the most devastating pathogen among all the rhizome rot pathogens. An effort was made for quick detection of bacterial wilt pathogen using PCR based molecular techniques. Phylogram results of four RS isolates Revealed that there are two major clusters, cluster A comprising all Ralstonia solanacearum species, A glance towards dendrogram reveals that there was not much diversity among the four isolates of RS. The fliC gene was amplified and an expected size of 400bp it was confirmed the Ralstonia solanacearum flagellin protein (fliC) gene. Fragment of fliC gene sequences of RS-1 (Haveri) and RS-8 (Shimoga) isolates showed 99 per cent similarity with NCBI published sequence of RS Strain MR11 plasmid mega plasmid flagellin protein (fliC) gene, partial cds (KF031064.1) and DSM9544 flagellin (fliC) gene, partial cds (AY192724.1) respectively. Dharwad isolate showed 98 per cent similarity with Ralstonia solanacearum strain RCR-226 flagellin protein (fliC) gene, partial cds (KC834785.1). While Uttara Kannada isolate showe 97 per cent similarity with RS flagellin (fliC) gene, complete cds (AF283285.1).
Ralstonia solanacearum, Bacterial wilt, quick detection, fliC gene, Polymerase chain reaction
© The Author(s) 2015. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.