ISSN: 0973-7510

E-ISSN: 2581-690X

Hai-han Zhang, Ting-lin Huang , Xiao Yang and Wei-xing Ma
1School of Environmental and Municipal Engineering, Xi’an University of Architecture & Technology, Xi’an – 710 055, China.
J Pure Appl Microbiol. 2013;7(3):2451-2458
© The Author(s). 2013
Received: 21/06/2013 | Accepted: 08/08/2013 | Published: 30/09/2013
Abstract

Soil microbial activity and community diversity in water level fluctuation zone (WLFZ) of the drinking water reservoir is still not well understood. In this context, soil dehydrogenase activity and bacterial community diversity of a drinking water reservoir was determined aiming to examine the water level fluctuation driving soil bacterial community activity and diversity across both vertical and longitudinal transects. BIOLOG method was employed to explore functional diversity of WLFZ soil bacterial community. The results shown that the highest dehydrogenase activity, 2.64 ìg TF/g.24h, was found in the top of WLFZ, which was 2.67 times higher than that of the bottom. Meanwhile, the average well color development (AWCD) 590nm, species richness and Shannon’s diversity of bacterial community associated with top of WLFZ was also significant higher than that of the bottom. The significant “site” and “WLFZ” revealed that the dehydrogenase activity, AWCD590nm and species richness varied among the sites within the WLFZ (P<0.01). However, there were no significant two-way interactions for different sites (P>0.05). Heatmap and principle component analyses (PCA) of bacterial community metabolic fingerprints suggested a significant discrimination soil bacterial community in the bottom, middle and top of three different sampling sites of WLFZ. The higher  discriminate  carbon  substrates utilization by WLFZ soil bacterial community were cellobiose, hydroxy benzoic acid, tween 80, arginine, threonine, pyruvic acid methyl ester, ketobutyric acid, malic acid, glucosaminic acid and glycogen. These founding demonstrated that water level draw down affect soil microbial activity and bacterial community functional diversity in the water level fluctuation zone of the JIN PEN drinking water reservoir.

Keywords

Bacterial community diversity, dehydrogenase activity, reservoir, water-level-fluctuating zone

Article Metrics

Article View: 722

Share This Article

© The Author(s) 2014. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.