ISSN: 0973-7510

E-ISSN: 2581-690X

Sandeep Acharya
Plant Physiology and Biochemistry Research Laboratory, Department of Botany, Tripura University, Suryamaninagar – 799 130, India.
J Pure Appl Microbiol. 2010;4(2):919-921
© The Author(s). 2010
Received: 05/01/2010 | Accepted: 19/02/2010| Published: 31/10/2010

For the authenticity of a new Rapid Antimicrobial Susceptibility Test (RAST) technique, antibacterial activity of Phyllanthus fraternus Webster., available in Tripura, was evaluated by a modified technique and  Disc-Diffusion (DD) method. Leaf extract was prepared in ethanol solvent to be  evaluated against  – Xanthomonas oryzae pv. oryzae, Xanthomonas campestris pv. campestris and Ralstonia soloanacearum. Compared to Streptomycin, the leaf  extract exhibited in vitro high antibacterial activity by the Silica Cotton ball technique which proves more economical than the conventional disc diffusion technique.


Phytopathogenic bacteria, Silica-Cotton Ball disc (SCB), Disc-Diffusion (DD) method

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