Groundnut bud necrosis disease caused by Peanut bud necrosis virus (PBNV) is a distinct member in the genus Tospovirus of the Bunyaviridae, and is the most economically important disease affecting groundnut crop. Bud necrosis infected groundnut plant samples were collected from ten groundnut growing districts of Karnataka were subjected to Direct antigen coated-enzyme linked immunosorbent assay (DAC-ELISA) and reverse transcription-polymerase chain reaction (RT-PCR) to detect GBNV. All the samples showed positive reactions with polyclonal antibodies of GBNV in DAC-ELISA and amplification of 831 bp GBNV coat protein gene by RT-PCR with degenerate primers.
Groundnut, GBNV-CP, DAC-ELISA, RT-PCR, Detection, Bud necrosis
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