ISSN: 0973-7510

E-ISSN: 2581-690X

K.M. Swamy and M.S. Patil
1Department of Plant Pathology, University of Agricultural Sciences, Dharwad – 580 005, India.
J Pure Appl Microbiol. 2014;8(4):3309-3312
© The Author(s). 2014
Received: 18/02/2014 | Accepted: 21/04/2014 | Published: 31/08/2014
Abstract

Groundnut bud necrosis disease caused by Peanut bud necrosis virus (PBNV) is a distinct member in the genus Tospovirus of the Bunyaviridae, and  is the most economically important disease affecting groundnut crop. Bud necrosis infected groundnut plant samples were collected from ten groundnut  growing districts of Karnataka were subjected to Direct antigen coated-enzyme linked immunosorbent assay (DAC-ELISA) and reverse transcription-polymerase chain reaction (RT-PCR) to detect GBNV. All the samples showed positive reactions with polyclonal antibodies of GBNV in DAC-ELISA and amplification of 831 bp GBNV coat protein gene by RT-PCR with degenerate primers.

Keywords

Groundnut, GBNV-CP, DAC-ELISA, RT-PCR, Detection, Bud necrosis

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