ISSN: 0973-7510
E-ISSN: 2581-690X
Capsicum chlorosis virus(CaCV) affecting chilli belongs to genus Tospovirus of the Bunyaviridae. This is economically important disease affecting chilli crop. Virus causes, stem necrosis and clorotic and necrotic spots on leaves. Serological detection showed CaCV positively reacted with GBNV Polyclonal antibodies in affected plants. Reverse transcription-polymerase chain reaction (RT-PCR) was used for studying the presence of CaCV in affected chilli plants from field sample in Northern Karnataka. Amplification of ~900 bp by RT-PCR with degenerate primers of L genes of Tospovirues revealed the positive reaction for CaCV infection in leaves of chilli.
CaCV, Tospovirus, RT-PCR, Detection, Serology, chilli
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