ISSN: 0973-7510

E-ISSN: 2581-690X

H.K. Sharma1 , S.K. Kotwal1, D.K. Singh2, M.A. Malik1, Arvind Kumar3, R. Katoch4 and M. Singh1
1Division of Veterinary Public Health and Epidemiology, Faculty of Veterinary Sciences and Animal Husbandry, SKUAST-Jammu, India.
2Brucella laboratory, Division of Veterinary Public Health, Indian Veterinary Research Institute, Izzatnagar, India.
3Division of Livestock Products Technology, SKUAST-Jammu, India.
4Division of Veterinary Parasitology, SKUAST-Jammu , R.S.Pura, Jammu and Kashmir-181102, India.
J. Pure Appl. Microbiol., 2016, 10 (3): 2277-2282
© The Author(s). 2016
Received: 20/03/2016 | Accepted: 19/05/2016 | Published: 30/09/2016

A study on Brucellosis in sheep from various parts of Jammu region was conducted. Serum samples (1085) from 13 different parts of Jammu region were collected and subjected to RBPT, mRBPT, STAT and I- ELISA tests. The overall seroprevalence of brucellosis in sheep was found to be 6.54%. The seroprevalence based on individual test was 150 (13.82%) (RBPT), 175 (16.13%) (STAT) and 251 (23.13%)by I-ELISA. The seropositivity for various tests was in following order: I-ELISA>STAT>mRBPT>RBPT. The female sheep from unorganized sector and older age group Sheep were found to be most vulnerable. The sensitivity of mRBPT and the kappa value as well as specificity of RBPT were found to be best for diagnosis of brucellosis in sheep. Among serological tests, I-ELISA was found to be most sensitive and detected maximum number of sera samples.


I-ELISA, Jammu, RBPT, Seroprevalence, Sheep brucellosis, STAT.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.