Selective Sclerotium cepivorum Growth Agar Media and Other Condition Factors Affecting

ISSN: 0973-7510

E-ISSN: 2581-690X

Selective Sclerotium cepivorum Growth Agar Media and Other Condition Factors Affecting

Mohamed Elsheshtawi¹, Ashraf A. Hatamleh², Amany M. Saad¹ and Abdallah M. Elgorban^3,4

¹Plant Pathology Department, College of Agriculture, Mansoura University, Mansoura, Egypt.

²Botany and Microbiology Department, College of Science, King Saud University,
P.O. Box 2455, Riyadh 11451, Saudi Arabia.

³Center of Excellence in Biotechnology Research, King Saud University,
P. O. Box 2455, Riyadh 11451, Saudi Arabia.

⁴Plant Pathology Institute, Agricultural Research Center, Giza, Egypt.

J Pure Appl Microbiol. 2014;8(4):2785-2790

Received: 18/02/2014 | Accepted: 21/04/2014 | Published: 31/08/2014

Abstract

New selective medium was used for S. cepivorum growth, forming sclerotia and sclerotial germination. Other affecting condition were evaluated such as pH degree and microelements. Onion bulb-extract at 50% in Potato dextrose agar (OE50PDA) was the best medium for the growth of S. cepivorum gave significant heavy mycelial growth and large numbers of sclerotia. The best growth of the fungus occurred at pH 6.0. Magnesium (Mg), sulfur (S), manganese (Mn) increased the mycelial growth regularity in all concentrations and formed large numbers of sclerotia and achieved significantly sclerotial germination.

Keywords

Sclerotium cepivorum, Selective Media, Onion extract, Sclerotia, pH degree

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