ISSN: 0973-7510

E-ISSN: 2581-690X

B.N. Meena , R.N. Pandey and Dama Ram
Department of Plant Pathology, B.A. College of Agriculture, AAU, Anand – 388 110, India.
J. Pure Appl. Microbiol., 2016, 10 (2): 1223-1232
© The Author(s). 2016
Received: 30/01/2016 | Accepted: 09/03/2016 | Published: 30/06/2016
Abstract

Seed bio priming of mungbean with the biocontrol agents i.e. Trichoderma viride, T. harzianum, T. virens and Pseudomonas fluorescens individually and in combination for their efficacy against root rot and blight diseases of mungbean caused by Macrophomina phaseolina and Rhizoctonia solani. A combination of T. viride + P. fluorescens promoted the seed germination, shoot length, root length, fresh shoot weight, dry shoot weight, plant height and most effective in reducing root rot incidence of the mungbean under poly-house conditions as compared with other single or combined treatments or the untreated control. The activity of the defense-related enzymes peroxidase, polyphenol oxidase and phenyl alanine ammonia lyase was significantly greater in mungbean root tissue treated with a talc based formulation containing T. viride + P. fluorescens than other treatments or the untreated control. Moreover, a combination of T. viride + P. fluorescens significantly increased phenol content, total soluble sugar, protein content in mungbean leaves under poly house conditions.

Keywords

Antagonistic activity, T. viride, T. harzianum, M. phaseolina, Rhizoctonia solani, bacterial bioagents, Defense-related enzymes peroxidase, Polyphenol oxidase and Phenyl alanine ammonia lyase.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.