ISSN: 0973-7510

E-ISSN: 2581-690X

Francisco J Bacame-Valenzuela1, Jorge A Rodriguez2, Juan C Mateos-Díaz2, Manuel Kirchmayr2, Elisa M Valenzuela-Soto1, Yolanda Reyes-Vidal1, Martin Esqueda1 and Ali Asaff1
1Centro de Investigación en Alimentación y Desarrollo, A.C., Apartado Postal 1735, Hermosillo, Sonora, 83340, México.
2Centro de Investigación Asistencia Tecnológica del Estado de Jalisco, Guadalajara, Jalisco, 44270, México.
J Pure Appl Microbiol. 2015;9(Spl. Edn. 1):131-138
© The Author(s). 2015
Received: 18/03/2015 | Accepted: 20/04/2015 | Published: 31/05/2015
Abstract

During last two decades, feruloyl esterases have been intensely studied for industrial or medical applications. Also screening of microbes from thermal and extreme environments as source of this kind of enzymes with improved properties is an active research field. In this context, screening and selection of fungal strains, for feruloyl esterase activity, were developed at Sonoran Desert, one of the hottest and driest regions of America. Sampling was carried out from soil and plant residues decomposing on soil. Samples were suspended in water, diluted, spread onto agar and cultured at 40 °C. Isolated strains were spread onto selective agar, choosing those with feruloyl esterase hydrolytic halo/colony diameters ratio>2. Selected strains were identified by molecular methods and their secretomes were assessed for methyl hydroxy cinnamates specificity. Twenty thermotolerant fungal strains, showing high feruloyl esterase activity, were found and identified as Aspergillus flavus (2), Aspergillus fumigatus (2), Aspergillus tamarii (2), Aspergillus terreus (11), Neurospora tetrasperma (1) and Phialophora alba (2). At least 10 of those shown 2.5 to 3.8 higher feruloyl esterase activities, than Aspergillus terreus ATCC1012, a mesophile strain. Secretomes exposed scarce specificity for substrates. Selected feruloyl esterases-producing strains show high potential to be used as biotechnological tools.

Keywords

Desert climate, Hydroxycinnamoyl esterases, Fungi

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© The Author(s) 2015. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.