ISSN: 0973-7510

E-ISSN: 2581-690X

P. Kaushik, Garima, Abhishek Chauhan and Pankaj Goyal
Department of Botany and Microbiology, Gurukul Kangri University, Hardwar – 249 404, India.
J Pure Appl Microbiol. 2009;3(1):169-174
© The Author(s). 2009
Received: 12/09/2008 | Accepted: 13/10/2008 | Published: 30/04/2009
Abstract

The aim of the present study was to investigate the antimicrobial activity of different organic extracts prepared from biomass and culture supernatant of Lyngbya majuscula against Escherichia coli, Bacillus cereus, Bacillus subtilis, Staphylococcus epidermidis, Enterococcus faecalis and a Yeast Candida albicans. Antimicrobial activity and minimum inhibitory concentration(s) of various extracts were evaluated by disc diffusion assay and broth micro dilution assay, respectively. Results indicated that this cyanobacterium excreted metabolites having broad spectrum antimicrobial activity. Methanolic extract was found to have significant activity against Bacillus subtilis with MIC value 512 µg/ml. HPTLC (high performance thin-layer chromatography) analysis at l 254 nm was performed for the separation of all the chemical constituents from the methanol extract. The antimicrobial activity was compared with standard antibiotic tetracycline and nystatin.

Keywords

Antimicrobial activity, Cyanobacteria, Lyngbya majuscula, HPTLC, Minimum inhibitory concentration

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