ISSN: 0973-7510

E-ISSN: 2581-690X

S.K. Shukla1 , A Chauhan1, S. Shukla2, M. Panigrahi1, B. Bhushan1, S. Maurya1, Sarvjeet1, R. Maurya1, S. Kumar1, C. Prakash3, V.K. Saxena1 and R.V. Singh1
1Division of Animal Genetics, Indian Veterinary Research Institute, Izatnagar, Bareilly – 243122, India.
2Department of Biosciences, Integral University, Lucknow, U.P., India.
3CADRAD, Indian Veterinary Research Institute, Izatnagar, Bareilly, India.
J. Pure Appl. Microbiol., 2016, 10 (2): 1527-1532
© The Author(s). 2016
Received: 23/12/2015 | Accepted: 12/03/2016 | Published: 30/06/2016

The intradermal tuberculin tests used worldwide for the diagnosis of bovine tuberculosis. The tuberculin skin test can provide a means for the early identification of Mycobacterium bovis infected cattle, thus ensuring their removal from an infected herd. We have done the skin test in three hundred numbers of cattle and results of skin testing after 72 hours revealed a pattern as depicted; positive cattle comprised of fifty six bovine tuberculosis infected animals from tuberculosis-infected herds, one hundred fifty one non-infected animals from tuberculosis-free herds and ninety three doubtful animals from tuberculosis-free herds. The aim of this study is, identification of bovine tuberculosis animal based on tuberculin skin test; which is considered as the primary method of bTB detection in India. The skin test is based on a differential delayed type hypersensitivity (DTH) response to intradermal injections of purified protein derivative (PPD) from M. bovis (PPD-B) and M. avium (PPD-A).


Bovine tuberculosis, Btb, Cattle, M. bovis.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.