Screening of Bacteria from Hydrocarbon Contaminated Soil in and around Mayiladuthurai with Reference to Biosurfactant Production and Bioremediation

ISSN: 0973-7510

E-ISSN: 2581-690X

J. Myla¹ , R. Chandrasekaran² and R. Saravana Muthu³

¹Department of Microbiology, ³Department of Botany, A.V.C. College (Autonomous),
Mannampandal, Mayiladuthurai – 609 305, India.

²Department of Botany and Microbiology, A.V.V.M. Sripushpam College, Poondi – Thanjavur – 613 503, India.

J Pure Appl Microbiol. 2010;4(1):343-348

Received: 20/01/2010 | Accepted: 09/03/2010| Published: 30/04/2010

Abstract

Petroleum is a complex mixture of hydrocarbons and other organic compounds. Oil pollution is hazardous to terrestrial and marine ecosystems. The contaminated soil samples were collected and analyzed for various physico – chemical parameters. The hydrocarbon degrading bacteria were isolated from contaminated soil. The selected organisms were tested for biosurfactant and BATH assay. The degradation potential of Pseudomonas, Flavobacterium, Klebsiella, Micrococcus and Bacillus sp were analyzed using BHA medium added with Petrol and Diesel as a substrate and was monitored by emulsification activity.

Keywords

Bacteria, Hydrocarbon, Bioremediation, Biosurfactant, Emulsification

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