Screening and Isolation of Phosphate Solubilizing Pseudomonas stutzeri (EGB3) from Gut of Earthworm (Eisenia foetida): Solubilization as Influenced by Organic Acids

ISSN: 0973-7510

E-ISSN: 2581-690X

Screening and Isolation of Phosphate Solubilizing Pseudomonas stutzeri (EGB₃) from Gut of Earthworm (Eisenia foetida): Solubilization as Influenced by Organic Acids

N.D. Prasanna , K.Vijayalakshmi, S.K. Shaheen, K. Pavana Jyotsna, G. Revathi, P. Jayalakshmi and P. Devika

Department of Applied Microbiology, Sri Padmavathi Mahila University, Tirupati – 517 502, India.

J Pure Appl Microbiol. 2010;4(2):717-723

Received: 14/04/2010 | Accepted: 30/05/2010| Published: 31/10/2010

Abstract

Crops absorb phosphorous in the form of soluble orthophosphate ion. The solubility of phosphate is inhibited by the presence of iron and aluminium in acidic soils and calcium in neutral and alkaline soils. This leads to fixation of phosphorous, making it not available to crop plants. The phosphate solubilizing bacteria (phosphobacteria) secretes organic acids like Gluconic acid, Oxalic acid, Acetic acid etc., which act on insoluble phosphates and convert the same into soluble form, thus providing phosphorous to plant. An experiment was conducted for the detection of organic acids using HPLC technique. Results clearly indicates that Gluconic acid and Oxalic acids were produced by Pseudomonas stutzeri in PKVS media.

Keywords

Phosphate solubilization, Pseudomonas stutzeri

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