To reduce the cost of Bacillus thuringiensis production, we used an industrial by-product made of molasses added to soybean flour. In addition, we scaled-up the production from a shake flask to a bioreactor. The results obtained with these cultures carried out in a 7-L bioreactor with 4-L of working volume were 5.68 ± 0.68 gDW/L, 8.9 x 109 spo/mL and 1.1 mg/mL of total protein. With these results, we produced 1.7-fold more biomass than a shake flask and a 15-fold higher spore count than a shake flask; statistically, we produced the same concentration as that obtained using a shake flask. Therefore, we have achieved a successful scale-up process.
KLa, Spore-crystal, Kinetic, Bacillus thuringiensis, Scale-up, Bioreactor.
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