ISSN: 0973-7510

E-ISSN: 2581-690X

Jesus A. Salazar-Magallon1, Karla T. Murillo-Alonso1, Laura Lina Garcia1, Guadalupe Pena-Chora2 and Victor M. Hernandez-Velazquez1
1Centro de Investigacion en Biotecnologia, Universidad Autonoma del Estado de Morelos, Av. Universidad 1001 Col. Chamilpa PO Box 62209, Cuernavaca, Morelos, Mexico.
2Centro de Investigaciones Bioligicas. Universidad Autonoma del Estado de Morelos, Av. Universidad 1001 Col. Chamilpa PO Box 62209, Cuernavaca, Morelos, Mexico.
J. Pure Appl. Microbiol., 2016, 10 (2): 871-878
© The Author(s). 2016
Received: 20/02/2016 | Accepted: 17/04/2016 | Published: 30/06/2016
Abstract

To reduce the cost of Bacillus thuringiensis production, we used an industrial by-product made of molasses added to soybean flour. In addition, we scaled-up the production from a shake flask to a bioreactor. The results obtained with these cultures carried out in a 7-L bioreactor with 4-L of working volume were 5.68 ± 0.68 gDW/L, 8.9 x 109 spo/mL and 1.1 mg/mL of total protein. With these results, we produced 1.7-fold more biomass than a shake flask and a 15-fold higher spore count than a shake flask; statistically, we produced the same concentration as that obtained using a shake flask. Therefore, we have achieved a successful scale-up process.

Keywords

KLa, Spore-crystal, Kinetic, Bacillus thuringiensis, Scale-up, Bioreactor.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.