ISSN: 0973-7510

E-ISSN: 2581-690X

A. Sheeba Christina and G. Swamidoss Daniel
Department of Biotechnology, PRIST University, Thanjavur – 614 904, India.
J Pure Appl Microbiol. 2010;4(1):247-250
© The Author(s). 2010
Received: 16/11/2009 | Accepted: 22/12/2009 | Published: 30/04/2010
Abstract

The present study was undertaken to evaluate the efficacy of Polymerase Chain Reaction (PCR) to detect M. tuberculosis in body fluids of patients with suspected Tuberculosis.  Suspected cases were chosen for study and controls included patients free of clinical TB. PCR was done on the clinical specimens and compared with AFB staining, culture on Lowenstein Jensen (LJ) medium and qualitative analysis by Dot ELISA. The sensitivity of PCR was found to be 100% in all 3 types of body fluids namely ascitic fluid, pleural fluid and CSF. PCR technique clearly becomes a valuable diagnostic tool for extrapulmonary TB in detecting fewer undetectable viable or non-viable MTB microbes.

Keywords

PCR, Tuberculosis, Mycobacterium tuberculosis, CSF, Acid Fast Bacilli, ELISA

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© The Author(s) 2010. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.