Review Separation and Purification of Proteins with Chromatography Applications

ISSN: 0973-7510

E-ISSN: 2581-690X

Huizhi Zhang¹, Daidi Fan¹ , Jianjun Deng¹, Xiaoxuan Ma¹, Chenghui Zhu¹ and Wenjiao Xue²

¹Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Xi’an, Shaanxi, China.

²Shaanxi Provincial Institute of Microbiology, No. 76 Xiying Road, Xi’an – 710 043, China.

J Pure Appl Microbiol. 2013;7(Spl. Edn.: April):07-12

Received: 03/03/2013 | Accepted: 14/04/2013 | Published: 30/04/2013

Abstract

The separation and purification of recombinant proteins are an important issue on bioprocessing. It not only contacts our lives, but associates with genetic engineering and expression. Because it is a junction of chemistry and biology, researchers should find a method to have a common effect on them for the separation and purification of protein. Though chromatography technology develops very quickly, it is difficult to meet requires of the separation and purification of protein. Despite these difficulties, chromatography, having been updated to solve the separation and purification of proteins constantly, which have been found widespread applications in the large scale purification of proteins from mammalian cells and microbial feedstocks in industrial bioprocessing. And others methodologies also are studied on the separation and purification of proteins.

Keywords

Review, protein, Chromatography, Bioprocessing

Download PDF

Article Metrics

Article View: 1076

JPAM.7.spl.edn.1.02 (306 downloads)

Share This Article

© The Author(s) 2013. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.