Two main species of gazelles are kept at King Khalid wildlife research centre.  Arabian mountain gazelle, Gazella gazella, locally known as Idmi and the Arabian sand gazelle, Gazella subgutturosa marica, locally known as Reem. Recently, lung infections of the captive gazelles have been recorded at the center. In addition, initial postmortem examination of the infected animals revealed the presence of supportive lesions and abscission in the gazelle’s lung. The objective of this study was to investigate Respiratory viruse in gazelles under captive at this Research Center. A total of 200 sera were collected from Idmi and Reem gazelles, each 100 samples between 2009 and 2010,  for evaluation of the humoral immune responses of Bovine Herpesvirus-1, ( BHV-1), Bovine Viral Diarrhea virus , (BVDV),  Bovine Respiratory Syncytial Virus,( BRSV), Bovine Adenovirus-3, (ADENO-3  ), Para- influenza virus-3 (PIV-3). An indirect ELISA test (Bio-X Respiratory penta kit, BIO K 028, Belgium) was used to detect antibody prevalence in these gazelles. This study has showed that these two Arabian gazelles (Idmi and Reem) were likely to be susceptible to infection by BRSV, ADENO-3 and PIV-3 as detected by Indirect ELISA. These animals could act as a source of virus infection during the infective stage of the viruses.