ISSN: 0973-7510
E-ISSN: 2581-690X
Ammonia-oxidizing bacteria were immobilized by sodium alginate and calcium chloride. The immobilization conditions and ammonia oxidation ability of the immobilized bacteria were investigated. The following immobilization conditions were found to be optimal: sodium alginate, 4.5%; calcium chloride, 2.0%; 2000 immobilized balls per 1000 immobilized medium; pH, 10; 110r/min and temperature, 30°C. The immobilized ammonia-oxidizing bacteria exhibited strong ammonia oxidation ability even after recycling for six times. The ammonia nitrogen removal rate of the immobilized ammonia-oxidizing bacteria reached 89.51% under the optimal immobilization conditions. When compared with non-immobilized ammonia-oxidizing, those immobilized by sodium alginate were superior with respect to preservation, recycle and ammonia oxidation ability.
Ammonia-oxidizing bacteria, Immobilization, Sodium alginate
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