The relationship between morphology of Schizochytrium sp. BCC 25505 and 25509 and production of docosahexaenoic (DHA) were studied in batch and pulsed-batch processes in optimized medium. In batch cultivation, the highest production by Schizochytrium sp. BCC 25505 (60-100% vegetative cells for 72 h) of biomass, DHA, and total fatty acid were 38.7 ± 0.14 g/L at 72 h, 5.9 ± 1.56 g/L at 63 h, and 13.03 ± 0.09 g/L at 54 h, respectively, whereas that of Schizochytrium sp. BCC 25509 (70-100% vegetative cells for 72 h) were 41.35 ± 0.46 g/L at 72 h, 12.09 ± 0.81 g/L at 69 h, and 26.32 ± 2.28 g/L at 66 h. In pulsed-batch cultivation, the highest production by Schizochytrium sp. BCC 25505 (57-86% vegetative cells) of biomass, DHA, and total fatty acid were 97.05 ± 1.63 g/L at 166 h, 20.73 ± 1.21 at 166 h, and 35.24 ± 3.27 g/L at 166 h, respectively, whereas that of Schizochytrium sp. BCC 25509 (25-94% vegetative cells) were 101.75 ± 2.19 g/L at 166 h, 20.0 ± 1.31 g/L at 143 h, and 36.48 ± 3.97 g/L at 118 h, respectively. Synchronous vegetative growth in high nitrogen content medium and in controlled high glucose supply for lipid accumulation enhanced efficiency of DHA production.
Schizochytrium, Docosahexaenoic acid, High cell density, Oleaginous microorganism, Polyunsaturated fatty acid, Thaustochytrid.
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