ISSN: 0973-7510

E-ISSN: 2581-690X

T.O. Femi-Ola
Department of Microbiology, University of Ado-Ekiti, P.M.B. 5363, Ado-Ekiti, Nigeria.
J Pure Appl Microbiol. 2008;2(1):181-186
© The Author(s). 2008
Received: 08/02/2008 | Accepted: 02/04/2008 | Published: 30/04/2008
Abstract

Isolation and characterization of mutations in the regulatory system controlling cellulase synthesis in Bacillus subtilis BS5 isolated from the hindgut of wood-eating termites Amitermes evuncifer Silvestri was carried out. Mutation was chemically induced using ethyl methane sulphonate (EMS). Derepression of the cellulase gene at various levels was achieved in these mutants resulting in differential rate of the enzyme biosynthesis. Three classes of mutants were identified with respect to their cellulase induction rate and level of cellulase production in the presence of high concentration of glucose (2% w/v).

Keywords

Cellulase, biosynthesis, Bacillus, mutation, termites

Article Metrics

Article View: 681

Share This Article

© The Author(s) 2008. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.