Hypoglycemic effect of Andrographis paniculata leaf extract and its glucose uptake inhibition effect in rat invivo models has been studied. Hypoglycemic  studies were carried out in glucose loaded and starch induced diabetic albino rats.Blood glucose levels were estimated at 10, 20, 30, 60, 120, 240 and 360 min after administration of  methanolic extract of A.paniculata by using One Touch digital glucose reader in both 200 and 500 mg/kg body weight.The blood glucose levels were significantly depleted in the animals administered with  aqueous leaf  extract of A.paniculata in both 200 and 500 mg/kg body weight. The aqueous extract of A. paniculata is very effective at regulating the activity of a-amylase and regulating blood glucose levels. There was a dose-response decrease in glucose absorption by the extract. The leaf extract of A. paniculata  has in vivo efficacy for inhibition of starch absorption and may prove beneficial in weight reduction in individuals consuming large amounts of starch. It also may inhibit starch-induced hyperglycemia in normal and diabetic subjects.