ISSN: 0973-7510

E-ISSN: 2581-690X

Z. P. Bhathena and Sachin S.Tawde
1Department of Microbiology, Bhavan’s College, Andheri-(W), Mumbai – 400 058, India.
J Pure Appl Microbiol. 2014;8(5):4189-4194
© The Author(s). 2014
Received: 28/04/2014 | Accepted: 15/06/2014 | Published: 31/10/2014
Abstract

A thermophilic alkalophilic organism isolated from the salt pan bed was used for the production of alkaline protease enzyme. The enzyme purification was carried out by using salt precipitation and DEAE cellulose chromatography to yield enzyme with a  specific activity of the 225U/mg .The enzyme had a  pH and temperature optima of 9.0 and 550C respectively and was capable of  recovering silver from exposed X-ray film at a recovery efficiency of 84.5%.. The recovered silver exhibited 98.10% purity which was better than the 70.5% recovery rate and 97.83% purity of silver recovered by traditional chemical method

Keywords

X ray films ,thermoalkalophilic, protease, extraction, Silver

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