ISSN: 0973-7510
E-ISSN: 2581-690X
Three alginate lyase producing strains of Klebsiella sp., and one strain of a Paenibacillus sp. were isolated from soil and water samples. The alginate lyase gene algK from Klebsiella sp. 20-3 and algM from Paenibacillus sp. 8-5 were cloned into Escherichia (E.) coli and expressed. The activity of recombinant AlgK (1640.1 U/mg) was 40 × higher than that of recombinant AlgM (36.7 U/mg). Sodium alginate was degraded by AlgK into alginate oligosaccharides. The AOs with a degree of polymerization value of 13.9 were found to stimulate the accumulation of phytoalexin in wounded soybean cotyledon. The 3 g/L concentration of AOs with DP 13.9 displayed the best effect on promoting germination and root development of rice seedlings, as well as a greater advantage for the rice seedlings to adapt to drought resistance and cadmium stress. The AOs with DP 13.9 were analysed by electrospray mass spectroscopy (ESI-MS), and the main component was trimer.
Alginate lyase, Alginate oligosaccharides, Plant resistance, Drought stress, Cadmium stress
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