ISSN: 0973-7510

E-ISSN: 2581-690X

Ramezan Ali Ataee1,3, Vahide Zare1 , Alishiri Gholam Hossein2, Davood Esmaeili1,3 and Mahdi Tata3
1Department of Medical Microbiology, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran.
2Department of Rheumatology, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran.
3Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
J. Pure Appl. Microbiol., 2016, 10 (2): 979-986
© The Author(s). 2016
Received: 10/02/2016 | Accepted: 31/03/2016 | Published: 30/06/2016
Abstract

Staphylococcal enterotoxins (superantigens) in Rheumatoid arthritis patients are considered. Nevertheless, there is unclear where is the origin of these superantigens in the SF of RA patients. This study aimed to assess mRNA encoding Staphylococcal enterotoxin B gene in Synovial fluid of the patients with RA. This experimental study, eighty Synovial fluids of patients with RA was assayed. The mRNA extraction carried out. Based on Staphylococcal enterotoxin B gene specific primers for RT Real Time PCR designed. Then, cDNA was synthesized and amplification by SYBR®Green Real Time RCR was down. The S. aureus strain ATCC 14458 as positive control was used. The results analyzed descriptively. The results indicated, using specific primers and probe for enterotoxin B could amplify the related cDNA gene. SYBR®Green Real Time RT RCR method has been outline that 38 cases (47.5%) were showed. In fact, the reverse transcriptase convert mRNA to cDNA for partially encoding staphylococcal enterotoxin B (superantigen) gene. In comparison positive and negative control, the results descriptively analyzed.The results showed, Real Time RT RCR were able to detect and characterized the mRNA producing enterotoxin B gene in Synovial fluid of 47.5% of the RA patients. Despite the finding may be discussed the direct role of enterotoxin B as a classic superantigen in the pathophysiology of RA disease. It may demonstrate the causative of disease and facilitate the rapid detection. However, more research is needed this finding could change the perspective approach treatment of the RA diseases.

Keywords

Real time RT RCR, Staphylococcal Enterotoxin B, Synovial fluid, Rheumatoid arthritis

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.